FastQCFastQC Report
Mon 27 Feb 2023
SRR3127891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064865
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483471.5774593660732201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143300.086.842571
CGTAGGG8250.076.3421552
ACGGGTA4200.076.0967564
TAGGGTA12700.073.2770544
AGGGATG54300.071.322555
ATAGGGC20450.070.78873
AGTAGGG52650.070.257062
TAGAGGG54100.069.937842
AAGGGTA24150.069.4796454
AGGGTAC14600.068.890665
AGATAGG18300.068.3885961
GTAGGGT14300.068.036323
AGGGAAT37100.067.776535
TAAGGGA27950.067.600643
ATAGGGA30100.067.300353
ATAACGG3400.066.422541
TAGGGAT22300.066.3914644
GTAGGGA27350.066.334053
AGGGATA33600.065.464525
ATGCGGG13800.065.393682
Produced by FastQC (version 0.11.5)