FastQCFastQC Report
Mon 27 Feb 2023
SRR3127890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539621
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290271.1429658204905377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89400.084.429921
AGTAGGG41800.071.647172
CGTAGGG7000.069.850762
AGGGCAT20250.069.62575
ATAGGGC16950.069.317633
ATAGCGG6250.069.2687451
AGGGATG36000.068.79895
TAGAGGG43600.068.581422
TAGGGCA13500.067.536944
GTAGGGA22700.067.2869643
GGGCGAT12400.066.705926
AGTAAGG13850.066.594111
TAAGGGA22550.066.275653
GTAAAGG20600.065.332361
AAGAGGG87350.065.180412
GAGGGAT31600.064.993174
ATAGGGA25750.064.974983
GAATAGG15150.064.917711
AAGGGAT41650.064.543784
TAGGGCG5100.064.506174
Produced by FastQC (version 0.11.5)