FastQCFastQC Report
Mon 27 Feb 2023
SRR3127888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994865
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385691.287837682165974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115350.085.411541
ATAGGGC19750.071.392823
TAGGGCA16900.070.361364
ATAGCGG6050.068.436031
AGGGCAT23400.068.292145
AGGGATG43550.068.1002665
CGTAGGG7850.067.655322
ATAAGGG45000.067.574822
AGTAGGG48200.067.183922
TAGAGGG53550.065.913382
AAGAGGG104150.065.569142
GAGGGAT38350.065.567534
AGTAAGG18600.065.515531
GAATAGG18050.065.1658861
AGGGAAT36900.065.088195
TAGCGGG16150.064.897132
CGAAGGG28950.064.77652
GTAGGGA25700.064.739683
AGAGGGC40400.064.567213
AGGGCGA17550.064.542775
Produced by FastQC (version 0.11.5)