FastQCFastQC Report
Mon 27 Feb 2023
SRR3127887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584700
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280401.08484543660773No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38240.14794753743181027No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27770.10743993500212791No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG25870.10008898518203274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88350.084.236411
AGGGATG46900.074.955835
ATAGGGA24250.071.320243
TAAGGGA21100.071.0534063
AGAGGGC36350.070.334943
GAGGGAT36300.070.172884
ATAGGGC15150.069.798583
ATAGAGG18400.069.0749361
ATAAGGG34800.068.640372
AGGGAAT27750.068.5912555
ACGGGTA2400.068.538324
AGGGTAC10650.068.400425
AGTAGGG44550.068.2891542
CGTAGGG6150.068.0471652
AAGGGTA16800.067.699084
GGTAAGG13700.067.3457261
GTAGGGA22300.067.2299043
TAGAGGG49700.066.8895342
AAGAGGG88350.066.793212
AGTAAGG16450.066.389081
Produced by FastQC (version 0.11.5)