FastQCFastQC Report
Mon 27 Feb 2023
SRR3127886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436229
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256251.051830513469793No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36390.14937019467381762No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG29240.12002155790773364No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG27030.11095016108912585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77300.084.331571
AGGGATG47000.075.394755
ATAGAGG17800.072.135511
AAGGGTA16350.069.2706154
GAGGGAT35650.069.2069244
ATAAGGG31850.069.1997152
ATAGGGA23500.068.1910553
TAGAGGG46700.067.824072
AGAGGGC33950.067.549313
CGTAGGG5800.067.24982
TAAGAGG17950.067.078291
TAAGGGA21250.067.007683
AGTAGGG38250.066.7128142
AGGGAAT25950.066.646615
ATAGGGC12800.066.452223
CTACGAC7500.066.422045
CGAAGGG24100.066.2983552
GAATAGG16000.066.140741
AAGAGGG85950.066.048342
GGTAAGG12750.066.031221
Produced by FastQC (version 0.11.5)