FastQCFastQC Report
Mon 27 Feb 2023
SRR3127884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677047
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230960.8627416702060143No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38860.14515994676223465No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG29030.10844038225701678Illumina PCR Primer Index 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76350.082.150811
TAAGGGA22150.072.140053
ATAGCGG4950.071.2929841
GTAGGGA22100.071.027333
CGTAGGG7700.070.831292
AGGGATG41500.070.325815
AGTAGGG46500.069.666532
TAGGGAT20700.069.019934
GAATAGG17050.068.993211
TAGAGGG47650.068.083842
ATAGGGC17000.068.00763
AGAGGGC41200.067.986133
ATAGGGA26500.067.7467963
GGTAAGG16750.066.8579251
AAGAGGG93000.066.784822
TATAGGG22250.066.141222
CGAAGGG28050.066.042152
TAGGGCA12450.065.682734
AGGGAAT28450.065.581225
AGATAGG16900.065.150821
Produced by FastQC (version 0.11.5)