FastQCFastQC Report
Mon 27 Feb 2023
SRR3127883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479011
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209050.8432798402266064No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33280.1342470848253598No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG33050.1333192954771076Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG27080.10923711108986608Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC25300.10205682830774047Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69100.081.4003751
ATAGCGG5150.073.0560151
ATAGGGA24050.070.152953
TAGGGCA11800.070.0966344
AGGGATG36350.069.818965
ATAGGGC16200.069.334513
GTAGGGA22600.069.039273
AGTAGGG45450.068.8663252
GAGGGAT33250.068.6927954
TAAGGGA20800.068.46143
TAGGGCG4000.068.145084
AGGGAAT29600.067.95735
TAGACGG3400.067.778261
GAATAGG16600.067.71161
TATAGGG20850.067.170222
GTACGGG4550.067.1380162
GGTAAGG13450.067.13551
ATAGAGG18300.066.818311
AGATAGG14750.066.638851
TAGGGTA7450.066.236834
Produced by FastQC (version 0.11.5)