FastQCFastQC Report
Mon 27 Feb 2023
SRR3127882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089297
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300750.9735224551087188No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44170.14297751235960804No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG31090.100637782641164Illumina PCR Primer Index 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98950.083.160561
CGTAGGG7700.074.467962
GTAGGGA27750.073.845473
AGTAGGG55400.071.094272
ATAGCGG5850.070.773431
AGGGATG45450.069.078515
GGTAAGG18050.068.552291
TAGGGAT24500.068.2942664
ATAGGGC18500.067.578763
AGATAGG20500.067.244561
TAGGGCA15600.067.186284
AGAGGGC46250.066.9690253
AGGGAAT34750.066.409015
TAGAGGG56250.066.2599262
GAGGGAT43350.066.028064
TAAGGGA26700.066.011613
TAGCGGG17550.065.612912
ATAGGGA31050.065.5430453
AAGAGGG103250.065.5044862
AGGGACT43350.065.052285
Produced by FastQC (version 0.11.5)