FastQCFastQC Report
Mon 27 Feb 2023
SRR3127881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675943
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515961.9281427145495997No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT44900.16779131693014387No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT37570.1403991041662696No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT34320.12825385294081376No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG30300.11323111142501913No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29080.10867197096500188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT161800.087.866411
ATAGCGG7100.072.9096451
ATAGGGA21100.072.834883
TAGACGG2750.071.8732451
ATAGGGC19800.070.258783
GAATAGG16850.070.101041
AGTAGGG41350.069.0288852
TAGGGCA15450.068.1386644
GTAGGGA17850.067.402483
TAAGGGA25250.067.3784943
AATAGGG40800.066.96282
ACGGGAT7100.066.85554
AGGGATG35050.066.641155
TAGGGTA7900.066.629274
GGTAAGG16550.066.537811
TAGAGGG45550.066.277222
GTAAGGG43100.066.116982
ATGTAGG8800.065.7768551
AAGAGGG92250.065.298082
AGGGACT30950.065.143455
Produced by FastQC (version 0.11.5)