FastQCFastQC Report
Mon 27 Feb 2023
SRR3127879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134576
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT667032.127975203025864No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT43900.1400508394117737No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT37140.11848492427684No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT35250.11245540066662923No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG34260.10929707877556645No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33560.10706392188289579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT201050.087.851731
ATAGGGC19450.074.910853
GAATAGG20550.072.1205141
AGTAGGG50300.070.4551852
AGTACGG4950.069.386951
GTACGGG8400.069.382832
ATAGGGA25350.068.044143
AATAGGG49150.067.800412
ATAGCGG6550.067.522221
AGATAGG17700.067.252341
ATAGGGT15400.067.14363
AGGGATG44450.066.717455
AAGGGAT52150.066.6028444
TAGGGCA16000.066.388234
AAGAGGG108050.066.2063142
ACGGGAT9250.066.054794
TAGAGGG55600.065.85252
GATAGGG44400.065.420682
AGTAAGG21950.065.162721
GGTAAGG18000.065.085911
Produced by FastQC (version 0.11.5)