FastQCFastQC Report
Mon 27 Feb 2023
SRR3127878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428225
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210450.8666824532322993No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57080.23506882599429627No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG31220.12857128149162456TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG25500.10501498007804055TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61950.082.8670041
CGTAGGG6850.076.884582
TAGGGCA12550.074.521164
CTACGAC12000.074.0202945
AGGGATG39950.073.4071045
GAATAGG16850.072.605861
ACGGGTA2550.071.877794
AGTAGGG37200.071.166762
ATAGGGC13500.070.6695863
ATAGGGA27200.070.495523
AAGGGTA18550.070.178834
GTAGGGT10850.069.73753
GAGGGAT37900.069.689514
ATAGCGG3200.069.1108251
TACGACC12800.069.026866
GGGCGAT9550.068.404036
TAGGGCG4350.068.0646444
TAGGGTA9400.067.995684
TAAGGGA22400.067.977823
ACGGGTC3100.066.705444
Produced by FastQC (version 0.11.5)