FastQCFastQC Report
Mon 27 Feb 2023
SRR3127877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258510
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191130.8462659009701087No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54520.24139808989112288No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG33760.14947908134123825TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG28000.12397554139676158TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25530.1130391275664044TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57500.082.2875751
AGGGATG37150.075.903175
CGTAGGG6600.074.76752
TAGGGCA10650.074.575294
AGTAGGG35150.073.002012
TAAGGGA18550.071.191793
GAGGGAT34150.071.009574
GTAGGGT10700.070.714683
AAGGGTA16100.070.639464
TAGGGCG3800.070.4935154
TACGACC11350.069.5634166
CTACGAC11500.069.064735
ACGGGTA2950.068.502174
ATAGGGC12650.068.358863
ATAGCGG4550.068.223921
AGGGTAT11750.067.595275
AGAGGGC30300.067.470533
AGGGAAT26950.067.3125465
ATAGGGA24300.067.1105353
TAGGGTA9150.066.7698064
Produced by FastQC (version 0.11.5)