FastQCFastQC Report
Mon 27 Feb 2023
SRR3127876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828202
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277920.9826737976990327No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC68760.2431226623840871No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG33970.12011164690499476TruSeq Adapter, Index 13 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29300.10359938929397547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77600.082.7000961
CGTAGGG9350.079.924142
ACGGGTA2150.074.325974
AGGGATG47500.073.518285
TAGGGCA15350.073.179544
TACGGGT3000.072.067053
TAAGGGA25450.071.100573
TACGACC13800.070.500376
ATAGGGA32350.070.028193
AGTAGGG44700.070.025982
CTACGAC13900.069.993185
GAGGGAT43300.069.9033664
AGGGTAC14000.069.4932255
TAGGGCG4300.068.8608254
TAGAGGG45650.068.4657442
ACGGGTC3800.068.026674
ATAGGGC16750.067.904843
GAATAGG20050.067.582081
TAGGGTA11800.067.313914
AAGGGTA21400.067.205964
Produced by FastQC (version 0.11.5)