FastQCFastQC Report
Mon 27 Feb 2023
SRR3127875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679082
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68781.01283791942652No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG34750.5117202340807149No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC30590.4504610636123473Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG30400.4476631688073016No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18030.265505491236699No Hit
AGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT12350.1818631623279663No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT10660.15697662432519194No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT9630.14180908932941824No Hit
ACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG9360.1378331335538271No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT8890.13091202535187207No Hit
GGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT8550.12590526622705359No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT7860.11574449035609838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGT254.920694E-793.994643
CGGGTAG1050.089.518695
CGTTTTT23650.088.528241
GGCCGAT750.087.728327
AGGGTAC3500.084.595165
GCGATGA401.035005E-982.245298
AGGGAAT18150.081.824525
GCTACGA3300.081.177184
CGCTACG3250.080.979983
TCCGCTA3350.080.0541
GGGAATG20900.079.8279656
TAGGGCG1650.079.753014
AGGGTAT4500.079.373245
ATAGCGG900.078.415471
AGTACGG600.078.415461
GGCGATG1500.078.328857
TAGGGCA2950.078.063344
GGCACTA850.077.407337
AGGGATG14200.077.11535
AAGGGCA9050.076.857484
Produced by FastQC (version 0.11.5)