FastQCFastQC Report
Mon 27 Feb 2023
SRR3127874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647299
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.9760558876191683No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG39130.604511979780596No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC33400.5159902919670817Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG32770.5062575409509361No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16660.2573771935380713No Hit
AGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT13120.20268840211401531No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT11720.18106006652258075No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT10830.16731062461088309No Hit
GGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT9520.14707268202175502No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT9480.14645472957628547No Hit
ACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG9310.14382843168303983No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT8910.13764890722834425No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT7500.1158660835255423No Hit
GGAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT7430.11478466674597057No Hit
GATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC7170.11076797585041842No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG7010.10829616606854019No Hit
ACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT6820.1053608919525598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAT4250.087.357175
GGGCGAT2100.087.278186
CGTTTTT21700.087.101981
AGGGAAT17150.086.8671345
AAGGGTA5300.084.2380144
GGGAATG20450.082.731256
TAGGGCG1500.081.459644
AATGCGG1100.081.2124561
CGTAGGG1050.080.5644762
AGGGTAC3400.078.7873155
AGGGATG12600.078.6995545
CTAACGG600.078.36291
GACCGAT301.4561665E-678.326588
GGACGAT301.4561665E-678.326588
AGGGCGC1200.078.326585
GAATAGG3700.077.515731
AAGGGCA8250.077.47214
CGGGTAT850.077.405085
TAAGGGA6750.076.585983
GGAATGA16650.075.362877
Produced by FastQC (version 0.11.5)