FastQCFastQC Report
Mon 27 Feb 2023
SRR3127872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2344644
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT693092.956056441830828No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT65080.27756879082709357No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT57820.24660460180735327No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT57030.24323522035754683No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48090.20510576445720544No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG38210.16296717113557538No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC27740.11831220432611518No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC26950.11494282287630873No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC25480.1086732143557828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT216050.089.4651261
AGGGATG41750.077.446825
ATAGGGC16350.074.448113
GAGGGAT30450.072.8494644
AGGGAAT26850.071.939795
GAATAGG13700.070.423341
AGTAGGG36200.069.618142
TAGGGCA11500.069.474074
AGGGTAC9250.068.590465
AGGGACT23100.068.156065
TAAGGGA21850.067.753353
AGTAAGG14550.067.60311
GGATGGC37900.066.9616557
AAGGGAT38550.066.929824
ATGAGGG36900.066.768422
AGATAGG11950.066.558291
TAGAGGG39350.066.5543752
GTAGGGT10600.066.505423
AGAGGGC34700.066.500313
TAGGGAT18550.066.3787464