FastQCFastQC Report
Mon 27 Feb 2023
SRR3127871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231635
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637932.858576783389757No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT72890.32662151292662106No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT62380.2795259977550092No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT62000.27782321033681584No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44830.2008841051516041No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG41660.186679273268254No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC31550.14137616590526675No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC30250.13555084052723676No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC28560.12797791753579774No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG23030.10319787958156239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT193650.089.55311
AGGGATG40150.075.147465
AGGGAAT25200.074.783995
ATAGGGC14350.071.3968663
CGTAGGG6500.070.8577352
GAGGGAT25650.070.357234
GAATAGG12950.070.099111
AGTAGGG33200.069.9299852
AAGGGAT38850.069.920044
AGTAAGG13050.069.561951
GTAGGGA14600.069.530523
TAGGGTC5950.069.5073244
GTAGGGT7950.069.16613
TAAGAGG14950.068.901381
AGAGGGC31150.068.6483
AGTACGG3500.068.537331
GTAGGGC11150.068.283343
TAGGGCA10350.067.656694
ATAGGGA20450.067.5660553
AGGGTAC8150.067.467265