FastQCFastQC Report
Mon 27 Feb 2023
SRR3127869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117000
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT849104.010864430798299No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT78140.3691072272083136No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT76410.36093528578176665No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT68220.32224846480869157No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG36310.17151629664619747No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC33100.15635333018422296No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC30150.14241851676901277No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC23550.11124232404345771TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT236850.089.6737751
GTAGGGT7400.073.67173
TAGGGTA6100.073.1927644
AGTAGGG30450.070.707492
TAGGGCA8950.069.8398064
ATAGGGC12250.069.82483
ACGGGTA2100.069.3772054
TAGAGGG34750.068.9926152
ATAGGGA15150.068.247143
AGGGATG26600.068.0226445
ATGTAGG6400.066.1667561
GAATAGG11250.066.0817951
ACGGGTC2000.065.796454
GTTTTTT326300.065.608972
TAAGGGA18000.065.2742543
TGTAGGG13550.065.2237552
AGGGAAT20800.065.073415
TATAGGG15400.065.0198752
AGGGACT19850.064.873075
GGTAAGG11900.064.844651