FastQCFastQC Report
Mon 27 Feb 2023
SRR3127868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988319
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT777043.9080248189551074No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT82650.4156777659922779No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT81520.40999457330539013No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT72210.363171100814306No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG40010.2012252561083005No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC35820.18015217880028306No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC32990.16591905021276768No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC24490.12316937070962959TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT220250.090.173061
ATAGGGC12200.073.189453
AGGGATG26500.072.711595
ATAGAGG13900.072.066991
AGAGGGC27100.071.793613
CGTTTCT12900.070.362171
TAGGGTA6500.070.131514
TAGAGGG35000.068.344652
TAAGGGA15550.068.30193
GAGGGAT20950.068.19374
GTAGGGC9750.067.480483
GAATAGG11550.067.1851961
TAGGGCA7800.066.8779754
AGATAGG10100.066.1208651
AGTAGGG27550.066.015232
TGTAGGG12200.065.8705142
GGTAAGG11500.065.8414841
AGGGAAT19000.065.795125
AGGGCAT12650.065.757975
GTTTTTT303350.065.4851762