Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127868.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1988319 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 77704 | 3.9080248189551074 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT | 8265 | 0.4156777659922779 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTT | 8152 | 0.40999457330539013 | No Hit |
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT | 7221 | 0.363171100814306 | No Hit |
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG | 4001 | 0.2012252561083005 | No Hit |
CGTTTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC | 3582 | 0.18015217880028306 | No Hit |
CGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC | 3299 | 0.16591905021276768 | No Hit |
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC | 2449 | 0.12316937070962959 | TruSeq Adapter, Index 15 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 22025 | 0.0 | 90.17306 | 1 |
ATAGGGC | 1220 | 0.0 | 73.18945 | 3 |
AGGGATG | 2650 | 0.0 | 72.71159 | 5 |
ATAGAGG | 1390 | 0.0 | 72.06699 | 1 |
AGAGGGC | 2710 | 0.0 | 71.79361 | 3 |
CGTTTCT | 1290 | 0.0 | 70.36217 | 1 |
TAGGGTA | 650 | 0.0 | 70.13151 | 4 |
TAGAGGG | 3500 | 0.0 | 68.34465 | 2 |
TAAGGGA | 1555 | 0.0 | 68.3019 | 3 |
GAGGGAT | 2095 | 0.0 | 68.1937 | 4 |
GTAGGGC | 975 | 0.0 | 67.48048 | 3 |
GAATAGG | 1155 | 0.0 | 67.185196 | 1 |
TAGGGCA | 780 | 0.0 | 66.877975 | 4 |
AGATAGG | 1010 | 0.0 | 66.120865 | 1 |
AGTAGGG | 2755 | 0.0 | 66.01523 | 2 |
TGTAGGG | 1220 | 0.0 | 65.870514 | 2 |
GGTAAGG | 1150 | 0.0 | 65.841484 | 1 |
AGGGAAT | 1900 | 0.0 | 65.79512 | 5 |
AGGGCAT | 1265 | 0.0 | 65.75797 | 5 |
GTTTTTT | 30335 | 0.0 | 65.485176 | 2 |