FastQCFastQC Report
Mon 27 Feb 2023
SRR3127866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677426
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434611.623238139914978No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28160.10517564257611602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118350.085.786491
GAATAGG16200.077.577051
ATAGGGC16250.076.644363
ATGTAGG8450.075.199351
TATAGGG20850.071.265552
AGTAGGG42650.071.221652
CGTAGGG6950.071.040022
TAGGGCA11600.070.903514
AGGGTAC13750.070.4128955
AGTACGG4450.068.75281
ATAGGGA21700.068.224213
ATAGAGG17150.068.065111
TAGGGTA7950.067.9858254
AATAGGG39350.067.754312
AGATAGG15900.066.607381
ATAGGGT13500.066.4947363
TGTAGGG19900.066.397522
GGTAAGG15250.066.3598861
AAGGGTA18500.066.306564
AAGGGGC81650.066.080514
Produced by FastQC (version 0.11.5)