FastQCFastQC Report
Mon 27 Feb 2023
SRR3127865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495800
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401761.6097443705425114No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG29590.118559179421428No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG28870.11567433287923712No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC26700.1069797259395785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110550.084.751421
GAATAGG13600.074.3559951
ATAGGGC16700.072.885783
ATAGAGG14050.071.639721
GGTAAGG15200.071.480051
AGTAGGG39300.069.716492
AGGGTAC14400.068.863365
AGATAGG12700.068.51481
CGTAGGG7300.068.2405552
ATGAGGG42150.068.235972
ATAGGGA20300.067.831453
TAGGGTA6450.067.761474
GAGGGTA14500.067.414754
ATAAGGG33950.067.136932
AATAGGG36450.066.91592
TATAGGG20200.066.771332
TAGGGCA12600.066.763954
AAGGGTA17550.066.4100954
AGGGAAT26100.066.083625
GGATAGG13250.066.025771
Produced by FastQC (version 0.11.5)