FastQCFastQC Report
Mon 27 Feb 2023
SRR3127864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098564
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT564961.8232962107608557No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32990.10646867387602774No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31530.10175681380148999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152550.085.903861
ATAGGGC18650.073.33463
GAATAGG20050.072.987031
AGTAGGG47350.069.68062
TATAGGG24250.069.5788962
GGTAAGG19000.067.3621
AAGGGTA20550.067.24044
ATAGGGA26950.066.9679953
TAGAGGG53950.066.905932
TAGGGCA13850.066.512174
AAGGGGC98150.066.465154
ATAGAGG20200.066.15561
AATAGGG47350.065.809462
AAGAGGG107750.065.6468662
ATAAGGG44500.065.271452
AGGGTAC17550.065.0775155
AGGGTAT16600.064.55485
ATGAGGG56900.064.510972
TGTAGGG23050.064.2294852
AGTAAGG19800.063.4520651
Produced by FastQC (version 0.11.5)