FastQCFastQC Report
Mon 27 Feb 2023
SRR3127863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882135
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349181.2115324230128013No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34550.11987641106332632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111550.084.197361
TAGGGTA10050.076.224364
ATAGGGC17600.072.632073
CGTAGGG8800.072.134312
ACGGGTA3750.071.435784
AGTAGGG48150.069.9209752
AGGGTAC15500.068.828195
GAGGGTA19550.068.7529754
TAGAGGG51200.068.6945652
TAGTAGG13300.067.929281
AAGGGTA22800.067.816174
AGGGATG39050.067.637495
AGGGTAT17100.067.3352055
TAGGGCA13050.066.984554
AGGGTAA17600.066.757425
GTAGGGA23800.066.151563
ATAGGGA27100.065.900163
AGGGCAT27350.065.813295
AGAGGGC41800.065.548763
GATAGGG42150.065.26032
Produced by FastQC (version 0.11.5)