FastQCFastQC Report
Mon 27 Feb 2023
SRR3127862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704394
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322861.1938349219825217No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31980.11825200026327526No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG30130.11141128104854543Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC29300.10834220161707207RNA PCR Primer, Index 41 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG28160.1041268395063737RNA PCR Primer, Index 41 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99900.084.940541
ACGGGTA3400.080.16624
ATAGGGC16300.071.213063
AGTAGGG42850.070.300352
GAATAGG16600.069.989481
ATAGGGT12100.069.90973
ATAGGGA25450.069.799823
AAGGGTA22150.069.589294
ATAAGGG37400.068.984422
CGTAGGG8650.068.4549262
AGGGTAC15400.067.745825
TAGGGCA12200.067.40944
TAGAGGG50150.067.3764652
TAGGGTA9950.067.066794
AGGGAAT32000.066.5270845
GATAGGG36250.066.505822
AGGGTAT16350.066.108965
GAGGGAT28450.065.9071964
AGGGATG36000.065.531715
AAGAGGG90350.065.48592
Produced by FastQC (version 0.11.5)