FastQCFastQC Report
Mon 27 Feb 2023
SRR3127861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369950
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454611.3490111129245241No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41660.12362201219602664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140850.084.9862751
ACGGGTA4750.078.167384
AGTAGGG55000.073.062672
TAGGGCA15550.072.841484
CGTAGGG9750.071.342642
ATAGGGA29750.070.7755363
GAGGGAT40450.070.063354
ATAAGGG47200.070.001192
AGGGATG49450.067.766555
TAGGGTA13050.067.347764
ATAGGGC20650.067.141973
ATAGGGT17200.066.9467853
TAGAGGG61150.066.483182
AAGGGTA28350.065.8157044
CGGGTAT4800.065.60335
GAATAGG19800.064.876291
AAGAGGG116150.064.783472
AGGGTAC18150.064.7374345
ACGGGAT7850.064.661564
GATAGGG47950.064.593512
Produced by FastQC (version 0.11.5)