FastQCFastQC Report
Mon 27 Feb 2023
SRR3127860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1961973
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225221.1479260927647832No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35300.17992092653670566No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG31260.1593294097319382No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC27370.13950242944219926No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG23820.1214083985865249No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC20470.10433374975088851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65550.082.63461
CGTAGGG5150.077.605832
AGGGATG40800.074.9882355
GAGGGAT29750.073.29994
AGGGAAT25800.072.863935
TAGGGCA10800.072.2364964
TAAGGGA19550.072.118523
ATAGAGG16550.071.373181
AGTAGGG32850.070.4226462
AGGGCAT17250.070.0190665
GGTAAGG11000.069.735551
GTACGGG4050.069.659262
TAGAGGG40650.069.517892
TAGGGTC7850.068.849454
AGGGTAC11750.068.395975
AAGAGGG67850.068.191132
GGATGGC34200.067.8848957
ATAAGGG26250.067.708812
ATAGCGG5150.067.621341
AGATAGG11000.067.596421
Produced by FastQC (version 0.11.5)