FastQCFastQC Report
Mon 27 Feb 2023
SRR3127859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818826
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203161.1169842524793465No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG32820.18044606795812243No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31460.17296871718350187No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC30110.16554634692928294No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG26030.1431142946054213No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18720.10292353419183584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57300.083.169851
ACGGGTA2900.077.783684
AGGGATG35450.074.371035
AGTAGGG29650.072.908542
TAAGGGA17500.072.774043
AGGGCAT14550.071.704635
GAGGGAT26200.070.8502044
GTAGGGT6750.070.317413
CGTAGGG5450.069.844762
AGGGAAT21850.069.471765
GTAGGGA13800.068.788773
ATAAGGG24700.068.6842
AAGGGTA15600.068.3827444
AGGGTAC9350.068.357245
ATAGGGA17550.068.282333
TAGGGCA7200.067.880674
AAGAGGG61850.067.775142
AGAGGGC25050.067.7243353
AAGGGAT33400.067.5367364
GGATGGC32650.067.362767
Produced by FastQC (version 0.11.5)