FastQCFastQC Report
Mon 27 Feb 2023
SRR3127858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280870
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291031.2759604887608675No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41580.18229885964566153No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG31270.1370968095507416No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC30260.13266867467238377No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG27950.12254096024762481No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22970.10070718629294961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86600.083.51311
AGGGATG49150.075.642475
AGTAGGG37950.074.806252
GTAGGGA18950.072.672683
ACGGGTA2800.072.181054
GAGGGAT35550.071.659284
TAGGGCA12100.071.473524
GTAGGGT10400.071.4062963
ATAAGGG33200.069.794562
GAATAGG15900.069.249611
AGGGAAT28600.069.187695
GGTAAGG14100.068.745891
TAGAGGG42350.068.698932
ATAGAGG19000.068.6001051
AGGGTAC12750.068.5670555
CGTAGGG6800.068.428822
TAAGGGA21950.068.307583
ATAGGGA24800.068.228143
GGATGGC42700.068.024267
AAGGGAT46400.067.86874
Produced by FastQC (version 0.11.5)