FastQCFastQC Report
Mon 27 Feb 2023
SRR3127857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393459
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119950.8608075300385588No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG41370.2968870989386842No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC34790.24966647744928266TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG33630.24134186940555838No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28850.20703874315641865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39050.083.633051
TAGCGGG6700.081.386962
AGGGATG25150.078.859245
AAGGGCA22800.075.443954
GTTACGG1250.075.2938461
TACGGGT1500.075.196583
CGTAGGG4200.074.988932
ATAGCGG2200.074.866041
AGGGCAT14550.074.6151665
AGGGAAT23500.074.596615
ACGGGTA1850.073.6723254
AGGGTAT11500.073.5618745
AAGGGAC15100.073.453614
CGAAGGG10800.073.1235352
TAGGGTA3600.073.107794
TAAGGGA12800.073.066993
GAAGGGC19750.071.865093
GGGTACG2500.071.436766
AGTAGGG22250.071.409892
AGAGGGC18200.071.271493
Produced by FastQC (version 0.11.5)