FastQCFastQC Report
Mon 27 Feb 2023
SRR3127856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293353
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108330.8375903562291191No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG46120.3565925157323639No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC38580.2982944331516608TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG37920.29319141796555154No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27050.20914630421856986No Hit
AGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT15710.1214672251117831TruSeq Adapter, Index 15 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT14740.11396733915644067No Hit
AGAGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT13230.1022922589579179No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT13140.10159639325072117TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36100.085.056671
CGGGTAT1650.082.603445
GGGTACG2550.079.252396
TAGCGGG5500.078.612652
TAGGGCA6700.078.5616464
GTACGGG1750.077.880242
TACGGGT1400.077.2088553
ATAGCGG1950.077.164541
AGGGATG24150.077.065885
GGGCGAT5400.076.590176
AATGCGG2050.075.694181
AGGGTAT9550.074.311785
CGTAGGG3350.072.9500962
AAGGGTA12400.072.769074
AGGGAAT20300.072.6972055
AAGGGCA19800.072.6312564
ACGGGTA2150.072.134464
TAAGGGA13100.072.1094363
AGAGGGC17550.072.034823
AAGGGAC14000.071.837814