FastQCFastQC Report
Mon 27 Feb 2023
SRR3127855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590243
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158710.9980235724980395No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG43100.27102776116606075No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC40140.25241425367066544TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG38330.24103234537111623No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35860.22550012796786403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG2650.086.976161
CGTTTTT48800.084.34091
CGGGTAT1500.081.463535
TACGGGT1500.078.335253
AGGGATG28850.077.5429465
TAGCGGG7150.076.910972
GGGTACG3050.075.5052956
AAGGGCA25900.074.403364
TAGGGCG2500.073.3217854
AGGGTAT12300.072.598835
GAAGGGC20150.072.542723
TAAGGGA16850.072.245093
AGTAGGG26850.071.945892
TGAAGGG24050.071.9185942
CGTAGGG4250.071.88412
GTAGGGC7850.071.848893
AGGGAAT27900.071.255325
AGGGTAC8550.070.909555
AGAGGGC20850.070.78353
TAGGGTC5250.070.725534
Produced by FastQC (version 0.11.5)