FastQCFastQC Report
Mon 27 Feb 2023
SRR3127854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471195
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421521.7057334609369152No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG27920.11298177602334095Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG26510.10727603446915358Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC26270.10630484441737703Illumina Single End Adapter 1 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT25010.10120609664555003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT122150.087.028031
ATAGGGC14250.073.876593
ATAGGGA19350.072.135513
ATAGCGG5400.071.458931
AGGGATG35450.070.661785
AGATAGG11550.069.263321
GGTAAGG14000.068.570691
AGTAAGG14800.068.0437851
GAGGGAT28750.068.003174
AGTAGGG39700.067.8640752
GTAGGGT8450.067.85423
AGAGGGC32600.067.756963
AGGGCAT19400.067.589025
ATGAGGG37550.067.241922
ATAGAGG15700.066.84081
AGGGAAT26850.065.8138355
AAGGGCA31250.065.570754
AAGGGGC68900.065.277934
AGGGACT27150.065.259715
CGTAGGG6650.065.04922
Produced by FastQC (version 0.11.5)