FastQCFastQC Report
Mon 27 Feb 2023
SRR3127853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319801
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384641.658073257145764No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG31310.13496847358889835Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG30480.13139058048513644Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC29080.12535558006915248Illumina Single End Adapter 1 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT26170.11281140063307155No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT26100.11250965061227235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111100.088.147131
GGTAAGG11650.071.8680041
ATAGGGA18700.071.368443
GAATAGG14050.070.63951
AGTAGGG37450.070.520262
AGTAAGG15950.069.5973741
TAGGGCA10550.069.486694
AGATAGG12050.069.4823461
AGGGAAT25450.069.24855
GAGGGAT27400.069.116854
CGTAGGG7100.068.834232
ATAGGGC14750.068.8162843
AGGGATG30950.068.4835
GTAGGGT9700.067.339913
TAGAGGG39400.067.030052
AATAGGG35200.066.8843462
GTAGGGA17500.066.8637543
AAGAGGG76300.066.146852
GTAGGGC11600.066.03273
AGAGGGC32650.065.775243
Produced by FastQC (version 0.11.5)