FastQCFastQC Report
Mon 27 Feb 2023
SRR3127852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903677
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555981.9147446496287295No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG31350.10796655413119295Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG29280.10083766204023381Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153100.085.8913351
ATAGGGA22400.072.176653
ATAGGGC17400.071.308453
TAGGGCA14800.070.180574
AGGGATA30150.068.901515
TAAGGGA24300.068.660733
ACGGGTA3050.067.801484
GTACGGG7500.067.67822
AGTAAGG17050.067.605881
GAGGGAT32400.067.3068544
AGGGATG39200.067.262125
AGATAGG14800.067.075441
AGGGAAT30300.067.009275
AGTAGGG44750.067.006042
AAGGGAT47450.066.7590264
TAGAGGG49550.066.3009642
AAGAGGG93900.066.218692
ATAGCGG5550.066.121761
AGAGGGC43400.065.949873
GGTAAGG13950.065.766261
Produced by FastQC (version 0.11.5)