FastQCFastQC Report
Mon 27 Feb 2023
SRR3127851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750100
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138480.7912690703388378No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC123100.7033883778069824No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG42520.24295754528312666No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG35980.20558825209988No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC33520.19153191246214502No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC33430.1910176561339352No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGGT25620.1463916347637278No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT21160.12090737672133021No Hit
AGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA17900.10227986972173018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47450.086.870391
GACCGAT1400.083.925588
CGTAGGG4750.082.170362
AGGGATG58350.081.914825
CGCTACG21000.081.463773
TAAGCGG4850.079.556561
GGATGGC46400.079.512257
CGGGATG10900.079.3366245
GCTACGA21450.079.316534
TAGGGCG3200.079.3096854
GATGACC29150.077.551278
CTACGAC21900.077.472135
TCCGCTA22150.077.3270651
AGGGAAT30350.077.117525
AAGGGAT47300.077.0057144
AGGGTAG6900.076.287155
CGACCAA21900.076.18458
GATGGCA45450.075.900498
AGGGCGA7400.075.578395
GGAATGC20150.075.570527
Produced by FastQC (version 0.11.5)