FastQCFastQC Report
Mon 27 Feb 2023
SRR3127848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772734
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620843.5021610687220983No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT46380.2616297763793101No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT44190.24927597710654842No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT42380.23906575944275904No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG24990.14096869581110308No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC20460.11541494663045895No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19570.11039445286207632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT180450.089.878231
ATAGGGC9650.071.592893
AGTAGGG28600.071.507252
TAGGGCA7100.070.166114
AGGGATG21200.068.945265
AGAGGGC26100.068.4262853
AGGGTAC6600.068.360815
TAGGGAT8700.068.066154
GAATAGG11350.067.985791
ATAGGGA11750.067.197213
TATAGGG11850.066.652712
TAGAGGG30600.066.065382
TAGGGTC4950.065.512444
ATGCGGG8800.065.178452
TAAGGGA13950.065.022393
AGATAGG7750.064.960931
GATAGGG21500.064.7262042
GTAGGGC9300.064.685493
GTTTTTT253500.064.595522
TAGTAGG7000.064.5272751
Produced by FastQC (version 0.11.5)