FastQCFastQC Report
Mon 27 Feb 2023
SRR3127845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206694
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274201.2425827958022273No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32530.14741509244145315No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG22100.10014981687538008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90800.086.473751
AGGGATG49850.077.026745
CGTAGGG5300.074.532222
AAGGGAT55900.073.987024
GAGGGAT43700.073.670534
TAGGGCA11900.072.670074
TAAGGGA24900.071.535983
ACGGGTA2250.071.0200654
TAGGGAT21400.070.93714
ATAGGGA26850.070.541623
AGTAGGG35800.070.408062
GTAGGGA21200.070.276173
TAGGGCG4600.069.476154
AAGGGTA20850.069.201744
AGGGATT36100.069.000685
AGGGATC22800.068.6426545
CGAGGGA14700.068.099973
GGATGGC36400.068.044647
AGAGGGC32850.067.8152853
AGATAGG15000.067.785461
Produced by FastQC (version 0.11.5)