FastQCFastQC Report
Mon 27 Feb 2023
SRR3127844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059571
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249591.211854313349722No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30440.14779776953550036No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG23770.1154123844237465No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG22890.11113964995622876No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC22130.10744956109791795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86000.086.7327961
CGTAGGG4600.079.68912
AGGGATG48300.076.672795
GTAGGGA18300.075.2451863
GAGGGAT37900.074.7722244
TAGGGCA12000.074.410554
GAATAGG12800.073.484991
ATGCGGG8950.072.988422
AAGGGAT51200.072.972524
AAGGGTA16050.072.90994
GTACGGG5350.072.90992
ATAGGGA23700.072.576323
AGGGTAC11800.071.290765
TAGGGAT21200.070.937574
AGGGTAT11050.070.600545
CGGGATG8150.069.773415
GGATGGC32850.069.673297
ATAGGGC14750.069.45873
TAAGGGA23000.069.0638963
AGGGAAT28950.068.667945
Produced by FastQC (version 0.11.5)