FastQCFastQC Report
Mon 27 Feb 2023
SRR3127842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282775
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205440.8999572888260998No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG33540.14692643821664422No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC29780.1304552573074438No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG29070.12734500772086604No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28840.12633746208014368No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23820.1043466833130729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73400.082.2442551
TACGGGT1800.075.719123
AGGGATG37850.074.2532355
CGTAGGG7250.073.9295652
ATAGGGC14800.072.0848853
TAGGGCG4400.071.565254
ATAGGGA26300.068.799453
ATAGCGG5000.068.6954351
CGAAGGG18700.068.639222
AAGGGAT38650.068.5819244
AGGGCGA14200.068.511285
TAGAGGG41100.068.29422
TAGGGCA12250.068.291084
ATGAGGG37500.067.453162
AGTAGGG37700.067.220032
GAGGGAT31550.066.437844
ACGGGAT8250.066.082144
GGGCGAT11200.065.8812266
TAGGGTC7000.065.79734
AGGGCAT17000.065.79735
Produced by FastQC (version 0.11.5)