FastQCFastQC Report
Mon 27 Feb 2023
SRR3127841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091509
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185990.8892622503656451No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG37000.17690576516763734No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC33440.15988456181637276No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG31870.15237801988898925No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24720.11819217607956743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60250.079.656741
CGTAGGG5650.076.526132
TAGGGCG4550.075.401824
GAATAGG11700.074.800841
ATAGCGG3400.073.345981
TAGGGTC6050.072.2433554
AGGGATG34500.071.6534355
ATAGGGA22900.071.213833
AGTAGGG32350.070.168662
TAGGGCA10600.070.052174
GTAGGGC9550.069.880393
AGGGAAT25550.069.713785
GAGGGAT26850.069.6641164
ATAGGGC11100.069.013653
AGGGCGA13250.068.810735
AAGGGAT37050.068.3708344
TAGGGAT15700.066.454394
AAGAGGG67500.066.283212
AGGGTAC8600.066.123725
GTACGGG4900.065.220362
Produced by FastQC (version 0.11.5)