FastQCFastQC Report
Mon 27 Feb 2023
SRR3127840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624375
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270751.0316742081447963No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG36250.1381281257442248No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC34160.13016432483924745No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34000.12955465587044535No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG32710.12463919980947845No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28390.10817813765182187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.081.7229841
CGTAGGG7300.075.326232
ATAGCGG6050.073.8751751
AGGGATG46300.073.492135
AGTACGG3150.073.1839451
TAGGGCA12700.072.5334
ATAGGGC14950.072.305263
GAGGGAT33050.070.3909764
ATAGGGA28850.069.560913
AGGGCAT21900.069.5319065
CGAAGGG23900.069.416082
GAATAGG17350.068.6042941
AGAGGGC38150.068.3725363
AAGGGAT46400.068.269254
GTACGGG6900.068.113652
AGGGAAT35900.067.682965
ATGAGGG45000.067.677732
GTAGGGA21150.066.664423
AGTAGGG40150.066.488422
TAGAGGG47800.066.171412
Produced by FastQC (version 0.11.5)