FastQCFastQC Report
Mon 27 Feb 2023
SRR3127839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423870
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45361.0701394295420765No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC21750.5131290254087338No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG21360.5039280911600255No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG18530.43716233750914196No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC8470.19982541817066554No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7100.16750418760469013No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT6620.1561799608370491No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT5980.14108099181352773No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG5690.1342392714747446No Hit
AAAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT5550.13093637200084932No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT5360.1264538655719914No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT5050.11914030245122324No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT4860.11465779602236534No Hit
GATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC4730.11159081793946257No Hit
GGAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT4410.10404133342770189No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG156.873748E-494.087381
ATTGCGG301.3153112E-894.087381
CACGGGT254.916237E-793.998593
GACCGAT156.8995956E-493.998598
ATTGCGT156.8995956E-493.998599
TAGGGCG353.583409E-1093.998584
ACGGGTA850.088.469264
CTACGAC2150.087.4405445
AGGGTAT3700.085.106835
TCCGCTA2300.083.860491
CGTTTTT16150.083.018271
GGTAAGG1550.081.947071
AGGGAAT10750.081.319715
TACGAGG700.080.6463241
CGGGTAC700.080.570215
GGGCGTT353.859168E-880.570216
GCTACGA2350.079.99884
ATAGCGG650.079.61241
AGGACGG650.079.61241
TAGCGGG1250.078.996082
Produced by FastQC (version 0.11.5)