FastQCFastQC Report
Mon 27 Feb 2023
SRR3127838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404554
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.982810700178468No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC23980.592751523900394No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG23850.5895381086332109No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG20690.5114273990616827No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7970.1970070744573036No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC7450.18415341338857114No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT7130.17624346811550498No Hit
AAAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT6640.16413136441612244No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT6450.15943483441023942No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT6430.15894046283067279No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG6170.15251363229630654No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT6090.15053614597804002No Hit
GATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC5700.14089590017649065No Hit
GGAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT5380.13298595490342452No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT5320.1315028401647246No Hit
GAAGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT5160.12754786752819156No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC4830.11939073646534208No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG4760.11766043593685886No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT4140.1023349169702932No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT4080.1008518022315933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG301.3182216E-894.05591
CGGGTAT201.836666E-593.997785
CGGGTAC700.093.997775
CGGGTAA301.3240424E-893.997775
GTCGTAC156.899603E-493.997778
ACGGGTA600.093.986154
AGGGTAC2050.087.119885
CGTTTTT13850.085.906661
ATAGCGG500.084.650321
TCCGCTA1800.083.605251
GTACGGC452.7284841E-1183.553588
TACGGGT452.7284841E-1183.543253
AGGGCGT850.082.939215
GGGTACG1150.081.737196
GAGGGTA2900.081.022554
TAAGCGG700.080.619341
GCGGGTA700.080.5595554
TAGGGCG353.8622602E-880.5595554
AGGGAAT9900.079.7556845
AGAGGGT3250.079.526753
Produced by FastQC (version 0.11.5)