FastQCFastQC Report
Mon 27 Feb 2023
SRR3127836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1852247
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624823.3733082034955384No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT47900.2586048189037423No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT46580.2514783395519064No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT39540.21347044967544826No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG25890.13977617455987243No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC22360.1207182411417052No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC20900.11283592307073517No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC19220.10376585844112585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT192000.089.343851
ATAGGGC11300.073.616743
ATAGGGA16800.071.6163943
AGGGATG26700.070.409385
ACGGGTA1350.069.6270454
ATGTAGG5250.068.1275251
AATAGGG27050.067.610872
AGAGGGC28900.066.8383563
AGGGTAC8050.066.556545
AGTAGGG29650.066.121932
GAATAGG12100.066.119861
GATAGGG25550.065.691852
AGGGAAT17450.065.17815
TAGCGGG9250.065.058262
TAGTAGG8400.064.990081
GGATAGG10500.064.9900741
ATAGCGG4000.064.709951
AGATAGG10050.064.622151
AAGAGGG62350.064.621732
TAGGGCG3900.063.8694274