FastQCFastQC Report
Mon 27 Feb 2023
SRR3127834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194079
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT792503.611993916353969No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT49310.2247412239942135No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT49300.22469564678391252No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT41570.18946446322124227No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG28090.12802638373549904No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC23750.10824587446486658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT236900.089.123341
ATAGGGC11300.071.123863
AGGGATG30750.070.003225
TAGAGGG38750.069.014162
AGTAAGG13250.067.814281
TAAGGGA17400.067.798813
GAGGGAT24850.067.142824
AGTAGGG35850.066.9932
GTACGGG4250.066.352912
AGGGAAT24900.066.0642245
ATAGGGA19650.065.776053
ACGGGAT5600.065.464264
AGAGGGC35300.065.107613
GAATAGG13250.064.618841
AATAGGG32800.064.481682
GTTTTTT329250.064.422452
TAGGGAT12800.064.257794
TATAGGG15500.063.9806142
GTAAGGG32150.063.8848842
AAGAGGG71950.063.820682
Produced by FastQC (version 0.11.5)