FastQCFastQC Report
Mon 27 Feb 2023
SRR3127833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272498
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.8774376325697802No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG11690.4289939742677011TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG11130.4084433647219429TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC10980.4029387371650434Illumina Single End Adapter 2 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT4210.15449654676364596No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4190.15376259642272605No Hit
TGAGCCAAACCTAGAAACAAACACAAAACACGAATAGTGAGAGACAAAAT4060.14899191920674648No Hit
GATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC3930.14422124199076689TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3920.14385426682030694No Hit
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3770.13834963926340746No Hit
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG3540.1299092103428282Illumina Single End Adapter 2 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT3500.12844130966098835No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC3490.12807433449052838No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT3190.11706507937672937No Hit
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3160.11596415386534947TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC2810.10312002289925062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA409.094947E-1293.9891744
CGGATGT156.899673E-493.9891749
TACGTAA156.899673E-493.9891749
TACGGGT353.583409E-1093.989173
CGGGAAT1550.087.9253545
GCTAGGG1250.086.4859162
GTAGGGT1250.086.470043
TAGGGCA900.083.545934
CGTTTTT8900.083.0071951
AGGGAAT7500.082.710475
AATCGGG401.031367E-982.255622
AGTAGGG3800.081.637162
GATTAGG750.081.5620651
TAGTAGG1450.081.129371
CGAAGGG2100.080.5769352
GGGTCTA353.8566213E-880.562146
ACGGGAT353.8566213E-880.562144
GGGAATG10050.080.428546
TACGAGG650.079.63161
AATGCGG600.078.425061
Produced by FastQC (version 0.11.5)