FastQCFastQC Report
Mon 27 Feb 2023
SRR3127832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268720
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.8213009824352486No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC13100.4874962786543614Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG12840.4778207799940458TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG12220.4547484370348318TruSeq Adapter, Index 22 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT4620.1719261685025305No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT4320.1607621315867818No Hit
GATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC4300.1600178624590652TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT4260.15852932420363203No Hit
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG4160.15480797856504913Illumina Single End Adapter 2 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4100.1525751711818994No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT3860.1436439416493004No Hit
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3830.14252753795772552TruSeq Adapter, Index 22 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC3800.14141113426615065No Hit
TGAGCCAAACCTAGAAACAAACACAAAACACGAATAGTGAGAGACAAAAT3550.13210777016969336No Hit
AGAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT3420.12727002083953556No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC3190.11871092587079488No Hit
GAAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT2980.11089610002977077No Hit
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT2790.10382554331646322TruSeq Adapter, Index 22 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT2750.10233700506103006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG156.8780273E-494.063131
CATACGG156.8780273E-494.063131
GGGTACG409.094947E-1293.993126
GATATCG201.8359906E-593.993128
GGTACCA156.898418E-493.993117
GACCGAT156.898418E-493.993118
TACGGGT254.9130176E-793.993113
GGACCGA156.898418E-493.993117
TACGTGA156.898418E-493.993113
ATGGGCG550.085.448294
CGTAGGG550.085.448292
GTAAGGG2850.084.0991062
TAACGGG452.7284841E-1183.549432
GGTAAGG1750.083.313061
CGAAGGG1750.083.2510452
AGGGAAT7950.082.761235
TCGAAGG401.02591E-982.305241
GACGGGT401.031367E-982.243973
CGGGAAT1850.081.291345
GGCAGAC353.855348E-880.565527
Produced by FastQC (version 0.11.5)