FastQCFastQC Report
Mon 27 Feb 2023
SRR3127831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320546
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32071.0004804302658588No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG12290.3834083095717931TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC11130.3472200557798257Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG10940.3412926693828655TruSeq Adapter, Index 22 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5300.1653428837046789No Hit
TGAGCCAAACCTAGAAACAAACACAAAACACGAATAGTGAGAGACAAAAT4880.15224024009034584No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT4390.13695382254029062No Hit
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3730.11636395400348157No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3670.11449214777286254No Hit
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG3550.11074853531162455Illumina Single End Adapter 2 (95% over 21bp)
GATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC3460.10794082596569603TruSeq Adapter, Index 22 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT3300.10294934268404535No Hit
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT3270.10201343956873585TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC3220.10045360104322001No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG156.8725925E-494.086041
GATCCTA156.898226E-493.997978
ATTCGAT156.898226E-493.9979794
TACGGGT254.9136725E-793.997973
ACGGGAT254.9136725E-793.997974
TAGGGAC700.093.997974
ATAGGGT1150.089.91113
TAGTAGG1950.089.2611161
CGGGAAT1450.087.515355
ACGGGTA600.086.16484
AGTAAGG2000.084.677441
CTAGCGG500.084.677441
TAGCGGG1000.084.5981752
TAAGGGA2700.083.553763
CGTTTTT11600.082.730831
ATAGCGG401.02591E-982.325291
AATACGG401.02591E-982.325291
AGGGAAT9600.082.248225
AACGAGG1150.081.813951
CTAGGGC1150.081.7373663
Produced by FastQC (version 0.11.5)