FastQCFastQC Report
Mon 27 Feb 2023
SRR3127830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006143
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109011.0834444010443844No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC80980.8048557709987546No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG39850.39606696066066155No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC34900.3468691826112193No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG33820.3361351219458864No Hit
AAAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT20280.20156180582680594No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18880.1876472827421152No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCACG17000.16896206602838762No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT13500.13417575831666076No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT13050.12970323303943873No Hit
GGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT12970.12890811743459926No Hit
TAGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT12550.12473376050919205No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG10330.10266930247489672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT33050.087.2485661
GCTACGA15300.086.316844
CGCTACG15400.086.061523
AGGGATG29800.085.164415
TCCGCTA16000.084.084381
CGGGAAT4550.083.6670155
ATAGCGG1350.083.6270451
TACGAGG1700.083.0121461
CTACGAC15950.082.7992255
CGACCAA15800.082.395468
GGATGGC26200.082.336437
GATGGCA24450.082.0785458
TAGGGTA4250.080.726214
AGGGTAC4500.080.419035
ACGACCA16150.080.3187947
AAGGGAT27250.080.1986544
ATGGCAC24450.080.1563349
GGGAATG23050.079.9274146
AAGGGCG4300.079.787544
GCATAGG2300.079.763851
Produced by FastQC (version 0.11.5)