FastQCFastQC Report
Mon 27 Feb 2023
SRR3127829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954168
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100511.053378440693882No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC75780.7941997635636492No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG44180.46302118704462947No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC39790.4170125177117657No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG37500.39301255124883666No Hit
AAAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT21420.22448876927333553No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC16970.17785127985847354No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCACG16740.1754408028774807No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT14250.14934476947455794No Hit
TAGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT14000.14672468579956569No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT13950.14620066906456725No Hit
GGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT13090.13718758122259395No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG11640.12199109590763892No Hit
GAACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT11120.11654132186365504No Hit
AGAGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT10950.11475966496466031No Hit
GGAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT9960.10438413361169101No Hit
AAAGCGGCTGTGCAGACACTGTCTCTTATACACATCTGACGCACGAGTCT9790.1026024767126963No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT9730.10197365663069816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT29700.086.161191
CGCTACG14900.084.218423
TCCGCTA15200.083.248781
AGGGATG27700.083.137795
AGGGAAT19650.082.755275
GGGAATG22100.082.725516
GGATGGC22800.082.453157
CGACCAA14850.081.970098
GCTACGA15200.081.9335254
GATGGCA21550.081.783588
CTACGAC15300.081.709465
ACGACCA14800.081.611917
CGAAGGG7450.081.37962
AGGGTAT5700.080.8040855
ACGGGTA1050.080.5642854
GGAATGC8750.078.957147
TACGAGG1500.078.400341
GCATTCG301.4562647E-678.33058
AAGGGCA12600.078.326394
ATGGCAC22150.078.082959